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反轉(zhuǎn)錄試劑盒 進(jìn)口試劑

反轉(zhuǎn)錄試劑盒 進(jìn)口試劑

簡(jiǎn)要描述:反轉(zhuǎn)錄試劑盒 進(jìn)口試劑
英文名稱: RevertAid First Strand cDNA Synthesis Kit
貨號(hào):K1622
品牌:Thermo*
規(guī)格:100 rxns
應(yīng)用范圍:molecular biology

產(chǎn)品型號(hào): K1622

所屬分類:生物試劑進(jìn)口

更新時(shí)間:2024-01-05

廠商性質(zhì):經(jīng)銷商

詳情介紹

反轉(zhuǎn)錄試劑盒 進(jìn)口試劑 *,質(zhì)量保證,現(xiàn)貨供應(yīng),1198元/盒
英文名稱: RevertAid First Strand cDNA Synthesis Kit
貨號(hào):K1622
品牌:Thermo*
規(guī)格:100 rxns
應(yīng)用范圍:molecular biology

反轉(zhuǎn)錄試劑盒 進(jìn)口試劑 產(chǎn)品描述:

Fermentas(MBI )自1975年成立至目前成功與Thermo合并以來一直為世界分子生物學(xué)科研領(lǐng)域提供優(yōu)質(zhì)和方便的產(chǎn)品。作為其產(chǎn)品之一,在中國(guó)用戶中的反映有口皆卑。試劑盒提供oligo(dT)18和隨機(jī)多態(tài)引物,同樣適合原核和真核生物的反轉(zhuǎn)錄。合成過程中,核酸酶抑制劑可減緩mRNA降解速度,以便能獲得全長(zhǎng)的cDNA*鏈,zui長(zhǎng)可以反轉(zhuǎn)錄9kb的片段。使用快捷、穩(wěn)定,在眾多品牌同類產(chǎn)品中其銷量屢見新高。

 

在M-MuLV逆轉(zhuǎn)錄酶的作用下,將mRNA轉(zhuǎn)錄為cDNA的*鏈。合成過程中,核酸酶抑制劑的加入可減緩mRNA降解速度,以便能獲得全長(zhǎng)的cDNA*鏈。

 試劑盒包括: 
M-MuLV逆轉(zhuǎn)錄酶
反轉(zhuǎn)錄Buffer
oligo(dT)18 
對(duì)照RNA和對(duì)照引物
RNA酶抑制劑
dNTP mix
隨機(jī)引物
DEPC處理水
 
 M1 - GeneRuler DNA Ladder Mix
1-6對(duì)應(yīng)擴(kuò)增片斷大小分別是:2023 bp,4564 bp、6149 bp、7850 bp、10092 bp、13305 bp。
M2 - Lambda pUC Mix Marker

產(chǎn)品英文描述:

Description

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5’-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

  • Full-length first strand cDNA up to 13 kb
  • Optimum reaction temperature 42°C
  • Complete kit – all the components for the RT reaction are included

Applications

  • First strand cDNA synthesis for RT-PCR and RT-qPCR
  • Construction of full length cDNA libraries
  • Antisense RNA synthesis

Includes

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.

The RevertAid RT Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Random Hexamer Primer, and nuclease-free water.

相關(guān)產(chǎn)品:

產(chǎn)品貨號(hào)*
反轉(zhuǎn)錄酶III Invitrogen試劑18080-0442000
MMLV逆轉(zhuǎn)錄酶 Promega試劑M1701220
反轉(zhuǎn)錄酶系統(tǒng)試劑盒 Promega試劑A35002755
Thermo反轉(zhuǎn)錄試劑盒K16221198


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