RPMI1640干粉培養(yǎng)基 Gibco試劑
簡要描述:RPMI1640干粉培養(yǎng)基 Gibco試劑英文名稱:RPMI 1640 Medium, Powder規(guī)格:10 x 1 L性狀:干粉培養(yǎng)基貨號:31800-022
產(chǎn)品型號: 31800-022
所屬分類:Gibco干粉培養(yǎng)基
更新時間:2024-01-05
廠商性質(zhì):經(jīng)銷商
RPMI1640干粉培養(yǎng)基 Gibco試劑 原裝正品,質(zhì)量保證,現(xiàn)貨供應(yīng) 290元/盒
英文名稱:RPMI 1640 Medium, Powder
規(guī)格:10 x 1 L
性狀:干粉培養(yǎng)基
貨號:31800-022
RPMI1640干粉培養(yǎng)基 Gibco試劑 產(chǎn)品描述:
描述
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With | Without |
• L-glutamine | • HEPES |
• Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
產(chǎn)品規(guī)格:
規(guī)格
Glutamine: | L-Glutamine |
---|---|
Phenol Red Indicator: | Phenol Red |
Form: | Powder |
HEPES Buffer: | No HEPES |
Serum Supplementation: | Standard Serum Supplementation |
Sodium Pyruvate Additive: | No Sodium Pyruvate |
Product Size: | 10 x 1 L |
Shipping Condition: | Room Temperature |
培養(yǎng)基配方
31800 - RPMI 1640, powder
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